Level 2
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There are 2 designated beneficiaries of the Trust but unclear if the RMD period is 5  or 10 years? The beneficiaries are the nieces of the deceased and only in their 40's? After reading the IRS Pub documentation I am still confused.
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Level 11
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IRAs left to an Estate or a Trust? You mention both. 

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Level 15
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This chart may help

From your statement - Died  before required beginning date & beneficiary of the IRA was a trust - With that "Take entire balance by end of 5th year following year of death"

Now the question is who owes the tax on the IRA? The trust was the beneficiary of the IRA, but the nieces are the beneficiaries of the trust. 

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